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TGF-β-mediated phosphorylation of Smad2 in NHLF cells Many of the effects of TGF-β are mediated through Smad2/3 pathways (Derynck et al., 2003). To determine the extent of Smad2 phosphorylation by
TGF-β, NHLF [...] Respiratory Medicine, Allergy and Rheumatic Diseases, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan, Phone: +81-6-879-3833, Fax: +81-6-879-3839, e-mail: hiro [...] (Belmont, MA) and MP Biochemicals (Irvine, CA), respectively. Antibodies against phospho-Smad2 and total Smad2 were obtained from Cell signaling technology (Beverly, MA). Fast Activated Cell-based ELISA …